Optimization of Umbilical Cord Mesenchymal Stromal Cell-Derived EVs Production Using EVs-Free Fetal Bovine Serum and Vegetal Peptone
🔬 Authors: C. Lezin, B. Lorenzini, S. Soave, S. Goulinet, H. Logtari, AC. Ponsen, G. Uzan, P. Mauduit
🏛 Institution: Inserm U1197, Paris-Saclay University, France
Abstract
Mesenchymal stromal cells (MSCs) were first identified in the 1960s and have since gained significant attention, particularly in cellular therapy. MSCs communicate with their microenvironment through both juxtracrine and paracrine pathways. Numerous studies indicate that their therapeutic effects are mainly mediated by their secretome, particularly extracellular vesicles (EVs). This discovery has paved the way for a novel approach known as acellular therapy.
MSCs-derived EVs are conventionally obtained either in chemically defined media or under nutrient-starved conditions. This study focuses on optimizing EV production conditions for therapeutic applications by modifying both the culture media composition and the culture platform.
1️⃣ EVs-free Fetal Bovine Serum (FBS) production: We utilize tangential flow filtration (TFF) to deplete FBS of existing EVs, ensuring a purified culture environment.
2️⃣ Vegetal peptone coating: Peptones derived from plant sources were applied as a culture surface coating to enhance MSCs growth and EVs yield.
Our optimized method significantly increased EVs production compared to traditional approaches. Furthermore, EVs obtained using this approach demonstrated superior performance in migration and wound healing assays. This technique represents a promising advancement for upscaling EVs production, which is essential for their clinical applications in therapeutic EV-based treatments.
🔍 Key Findings
✅ EVs-free FBS + vegetal peptone coating enhances EVs yield significantly
✅ Optimized culture conditions improve EVs function in migration and wound healing assays
✅ Scalable production strategy, meeting clinical EV manufacturing requirements
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This research is sourced from Organotechnie and is provided for academic and technical reference only.