OPTIMISATION OF PROTEIN PRODUCTION IN HEK293 CELLS WITH DIFFERENT PEPTONES
🔬 Research Author: Gizem Yalkin / R&D Department
📄 Research Source: Organotechnie
Abstract
Mammalian cells are the natural source of many therapeutic proteins and are widely used as the most elaborated platform for the production of recombinant proteins with clinical applications.
For more than 15 years, Organotechnie’s Tryptone N1 has been widely documented to favor high titer protein expression post-transfection.
In this study, HEK293 cells were used to optimize IL-4 (Interleukin-4) expression with different sources of peptones, comparing Vegetal Peptones with Tryptone N1 as a control.
Key Findings
The study shows that Peptones derived from Pea, Guar, and Soy proteins significantly enhanced protein production, achieving an increase of approximately 150% compared to the control group (Tryptone N1).
The plant-based peptones used in the study:
- P112 (Pea Peptone)
- G115 (Guar Peptone)
- S204 (Soy Peptone)
🔍 Research Contribution
This study demonstrates that plant-based peptones can effectively enhance recombinant protein expression in HEK293 cells and serve as a viable alternative to animal-derived peptones in biopharmaceutical and cell culture applications.
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This research is sourced from Organotechnie and is provided for academic and technical reference only.