Investigating the Proliferative and Serum-Reducing Effects of Various Protein Hydrolysates on Vero Cells for ATMP Bioproduction
🔬 Authors: Chloé Lezin, Georges Uzan, Mohamed Essameldin Abdelgawad
🏛 Institutions:
- Inserm U1197, Paris-Saclay University, France
- Organotechnie, R&D Department, France
- Biochemistry Division, Chemistry Department, Faculty of Science, Helwan University, Cairo, Egypt
Abstract
Fetal bovine serum (FBS) is widely used in cell culture as a key supplement due to its ability to provide essential nutrients, growth factors, hormones, binding/transport proteins, and attachment factors. However, reducing or replacing FBS is a critical challenge in modern bioproduction, particularly for Vero cells, which are widely utilized in Advanced Therapy Medicinal Products (ATMP) manufacturing.
In this study, we explored whether peptones could serve as an alternative to FBS in expansion media for Vero cells. Results indicate that both the raw material source and the manufacturing process of peptones significantly affect Vero cell proliferation.
Key Findings
✅ Serum reduction by 50%: By supplementing media with 0.5 g/L of peptones, serum levels were successfully reduced from 10% to 5% while maintaining the same proliferation efficiency.
✅ Peptone source matters: Peptones derived from casein, wheat, and soy exhibited different impacts on Vero cell growth.
✅ Media optimization is crucial: A newly developed BMBD145 Av medium yielded superior results compared to the standard DMEM + 10% FBS control medium.
✅ Processing method influences performance:
- Gamma irradiation & ultrafiltration positively impacted the performance of wheat-based peptones.
- Casein-based peptones showed opposite effects under similar treatments.
✅ Optimized peptone-media combinations performed better than using peptones in generic culture media.
This study highlights the batch-to-batch consistency of casein-based peptones and suggests that strategic selection of peptones and optimized media can significantly improve serum-free or reduced-serum culture strategies for ATMP production.
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This research is sourced from Organotechnie and is provided for academic and technical reference only.